Study of Genetic Diversity of Berseem Accessions (Trifolium Alexandrinum L. SPP) With RAPD Markers

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Year:
2016
Type of Publication:
Article
Keywords:
Trifolium Alexandrinum, Berseem, Genetic Diversity, T Berytheum
Authors:
ROUZ, S.; JEDDI, F. BEN; ZOUAGHI, M.
Journal:
IJRAS
Volume:
3
Number:
3
Pages:
168-170
Month:
May
ISSN:
2348-3997
Note:
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. CC BY-NC-SA 4.0 Creative Commons License: https://creativecommons.org/licenses/by-nc-sa/4.0/
Abstract:
This study aimed to screening genetic diversity and ancestral relationship between Tunisian berseem accessions. The analysis of the genetic diversity of 29 accessions of berseem kept and recorded in the fodder index of Tunisian National Institute of Agronomy has been conducted via RAPD markers. Several prompts have been tested, but 5 only were selected for their reproductive potential and their high polymorphism rates. A total of 388 reproducible bands were obtained during PCR-based amplifications. The bands have a size ranging between 200 and 2000 pb; though, most of the bands are in the range of 200 to 600 pb. The correlations between the accessions have been evaluated by means of analyses according to the UPGMA method. The extent of dissimilarity between the accessions varies from 16 to 100%, which represents a high polymorphism level. The dendrogram, worked out via the Treecon software, reveals seven sets of accessions at the 60% threshold. T. berytheum, ancestor of berseem, clearly emerged from the other accessions. It is of interest for intensive crop-growing systems and may be used as parent in selection programs. T. meironense is genetically very close to the T. alexandrinum var serotinum. Three sets are generated by T. alexandrinum and T. berytheum crosses. The present study showed a high genetic diversity of studied berseem accession. Showed results can be used as a pathway for preservation and productivity improvement under different agricultural systems.

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