Influence of Genotype, Explant Type and Hormonal Composition of Culture Medium on in Vitro Callogenesis of Citrus Species

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Year:
2016
Type of Publication:
Article
Keywords:
Citrus, Callogenesis, Explant, Friable, Phytohormones
Authors:
Z, ELMANDOURI F; O, CHETTO; A, FADLI; A, TALHA; R, BENKIRANE; H, BENYAHIA
Journal:
IJRAS
Volume:
3
Number:
6
Pages:
296-303
Month:
November
ISSN:
2348-3997
Note:
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. CC BY-NC-SA 4.0 Creative Commons License: https://creativecommons.org/licenses/by-nc-sa/4.0/
Abstract:
In order to establish optimum conditions for callogenesis of citrus species, the effects of some factors, namely the genotype, explant type and hormonal composition of culture medium on callus induction were studied using eight cultivars: Amblycarpa (C. amblycarpa (Hassk.) Ochse), Cleopatra mandarin (C. reticulata blanco), Carrizo citrange (Citrus sinensis (L.) Osbeck X Poncirus trifoliata (L.) Raf.), Hamlin orange (Citrus sinensis (L.) Osb.), Rangpur lime (Citrus x limonia Osbeck), trifoliate orange (Poncirus. trifoliata), Troyer citrange (C. sinensis X P. trifoliata) and Volkamer lemon (Citrus Volkameriana). The present work focused particularly on the ability to induce friable calluses from three types of flower explants: styles, stigmas and ovules. These were sampled from mature trees before anthesis and cultivated on MS or MT medium (Murashige and Skoog) supplemented with malt extract, saccharose, agar and phytohormones including 2,4-dichlorophenoxyacetic acid (2-4D), 6-benzylaminopurine (BAP) and Kinetin (Kn). Callogenesis, which started after 15 days of dark incubation, was shown to be the most successful when using styles of Troyer and Carrizo citranges as explants and MS medium containing the hormone combination: 1 mg.l-1 2, 4-D+0.5 mg.l-1 BAP. However, taken together, our results suggest that ovules are more suitable explants for callus induction than styles and stigmas since these resulted generally in higher callogenesis rates in all the media used. The interactive effects between studied factors and their influence on callus color and texture are also discussed in this paper.

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