Methodology for Buckwheat Germination Test

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Year:
2022
Type of Publication:
Article
Keywords:
Fagopyrum esculentum, Rules for Seed Analysis, Germination Speed Index, Mean Germination Time
Authors:
Pagliarini, Maximiliano Kawahata; Pontim, Bruno Cezar Alvaro; da Silva Ribeiro, Fabio; Gordin, Carla Regina Baptista; de Almeida Monaco-Mello, Kamila; dos Santos Zomerfeld, Patricia; de Souza, Luiz Carlos Ferreira
Journal:
IJRAS
Volume:
9
Number:
2
Pages:
49-58
Month:
March
ISSN:
2348-3997
Abstract:
Buckwheat (Fagopyrum esculentum) despite its name, it is not related to common wheat, this comparison is due to the use of its grains that may be incorporated, in human nutrition in flours form. Among the factors responsible for the success of buckwheat cultivation, the quality of seeds is fundamental, especially regarding to germination and vigour levels. In Brazil, germination tests are guided by the Rules for Seed Analysis, a publication made by the Ministry of Agriculture, Livestock and Supply, however, not all plant species have already had a determined methodology. Thus, the objective of this research was to determine the suitable methodology protocol for conducting the buckwheat seed germination test. The experiment analyses were performed at the Laboratory of research, teaching and extension support at experimental farm of Agrarian Sciences of Grand Dourados Federal University (UFGD), in the municipality of Dourados, Mato Grosso do Sul state, Brazil, with buckwheat seed produced at the same farm. The experimental design used was completely randomized in a 4×3 factorial scheme, with four temperatures, three substrates and four replications with 50 seeds each. The temperatures were: 15°C, 25°C, 30°C and 35°C and the substrates were: on paper, between paper and paper roll. The treatments were placed to germinate in B.O.D with constant light. The counting of germinated seeds occurred daily until the germination stabilization date, verified when the number of normal seedlings remained stable for three successive counting days. The characteristics evaluated were: First germination count, percentage of germination, germination speed index, mean germination time, mean germination speed, seedling length and synchronization index. For buckwheat (Fagopyrum esculentum) germination test in the laboratory, the substrates on paper and between paper at temperatures of 25°C and 30°C are indicated with constant light. Furthermore, the first count must occur at four days and the final evaluation of the germination percentage at eight days after sowing.

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