Physiological and Biochemical Characters of Ralstonia solanacearum

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Year:
2014
Type of Publication:
Article
Keywords:
Chilli, Ralstonia, Wilt, Biochemical Characters
Authors:
Pawaskar, Jayesh; Joshi, M. S.; Navathe, Sudhir; Agale, R. C.
Journal:
IJRAS
Volume:
1
Number:
6
Pages:
357-360
Month:
November
Note:
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. CC BY-NC-SA 4.0 Creative Commons License: https://creativecommons.org/licenses/by-nc-sa/4.0/
Abstract:
Ralstonia solanacearum causes bacterial wilt of solanaceous crop plants a most devastating disease in humid tropic. Bacterium from roots of wilted plants was isolated on nutrient agar medium. It was identified as Ralstonia solanacearum on the basis of morphology as well as molecular marker basis. R. solanacearum was small straight rod shaped, measuring 1.5–3.12µ X 0.25–2.5µ and Gram negative in reaction with KOH positive test. The colonies of R. solanacearum on nutrient agar medium were smooth circular, raised and dirty white. The bacterium was visible in the form of thin pellicle on the surface of nutrient broth in 24 hrs. Later on, the growth became a little thick and medium became turbid with putrefactive odour. The bacterium was positive in acid and gas production tests and negatively responded to starch hydrolysis, hydrogen sulphide production and cellulose decomposition tests.

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