In VITRO Microtuberization of Two Potato Cultivars Under Sea Water Stress
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- Microtuber Production, Nodal Cuttings, Salinity, Solanum Tuberosum
- EL-Kazzaz, Ahmed A; Ebad, Fawzia A; EL-Sadek, Marwa E Abd
- This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. CC BY-NC-SA 4.0 Creative Commons License: https://creativecommons.org/licenses/by-nc-sa/4.0/
- Plant nodal cuttings as single node explants from previously selected plantlets on innovative exchangeable cycles of selection in vitro of two potato verities named Lady Rosetta and Cara were used for induction of microtubers in vitro. The nodal cuttings were cultured on solidified MS medium free of plant growth regulators and supplemented with 8% sucrose and two doses of sea waters (8000 and 9000 ppm for Lady Rosetta and 7000 and 8000 ppm for Cara). The results cleared that the tested plantlets under higher saline concentration (9000 ppm for potato lady Rosetta and 8000 ppm for potato Cara) gave higher numbers of microtubers (6.7 and 6 respectively) as compared to the control plantlets which gave (5.7 and 5.3 respectively) and grown on medium free of sea water. However, data cleared that the fresh matter and microtuber diameter of both tested cultivar showed a slight increase over the control. The presented work brings an efficient easy protocol for production of potato microtubers in vitro under saline conditions.
Full text: IJRAS_388_FINAL.pdf