Induction of Somatic Embryogenesis in Jatropha Curcas L.
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- Callus, Proembryos, Regeneration, Somatic Embryogenesis
- Laguna, Graciela; Molina, Maritza Vacca; Padilla, Alejandra; Aviles, Zulma; Bonomo, Cristina; Carrizo, Adolfo; Martinez, Roberto
- Jatropha curcas L. is a drought resistant species and can be grown in marginal areas. Because of its high content of oil in the seeds, it is interesting for the production of biodisel. Conventional methods of vegetative and sexual propagation are deficient due to low multiplication rates. Somatic embryogenesis represents an applicable regeneration pathway. The objective of this work is to introduce the metodology aplied in the induction of embryogenesis in three types of explants, for which ten treatments were evaluated with 2,4-D and ANA. Statistically significant differences were detected in the responses according to explants types. All treatments with growth regulator formed callused masses. The highest proliferation of callus occurred in foliar segments with 2.4 D to 107.41 μM, and in explants from embryos and cotyledonary leaves the concentrations were 67.87 and 135.74 μM respectively. Proembrional stages were observed in callus from leaf segments with 2.4 D to 107.41 μM
Full text: IJRAS_741_FINAL.pdf