Callus Induction and Regeneration in Commercial Rice Cultivars of Tamil Nadu
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- Callus, Matured Embryo, Rice, Regeneration, Somaclone
- Abiramasundari, N.; Shanthi, P.; Kumar, D. Sassi; Saraswathi, R.
- This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. CC BY-NC-SA 4.0 Creative Commons License: https://creativecommons.org/licenses/by-nc-sa/4.0/
- Ten commercial rice cultivars belonging to subspecies indica were compared for its capacity in callus formation and regeneration. Among the different media composition, Ii was found that the dark condition with optimum level of 2, 4 D (2mg/l) + Kin (0.5mg/l) was the best to get better and early callus induction frequency followed by 1.5 and 2.5 mg/2,4 D/l. It was found that the dark condition with optimum level of 2, 4, D (2mg/l) + Kin (0.5mg/l) was the best to get better callus induction frequency. Among the different varieties tested the varieties viz., ADT 37, ADT 43 and ADT (R) 47 responded better for callus induction than the others ADT 44, ADT (R) 48, TNAU Rice ADT 49 and ADT (R)45. The genotype TNAU Rice ADT 49 had very low per cent of callus induction frequency. Among the rice genotypes ADT 43 only produces shoot in MS + 1.5mg/l BAP+1.5 mg/l Kin+0.5 mg/l NAA media composition. The varieties ADT (R) 46, ADT (R) 47, ADT (R) 48 and ADT 37 have produced only the green spots in the above combination.
Full text: IJRAS_36_Final.pdf